Challenge Runs are special game modes with predefined rules and restrictions. These can range from starting with specific Jokers, having altered shop contents, or imposing unique scoring conditions. Each challenge run offers a distinct experience and often requires a different approach than a standard run. Successfully completing them can unlock new Jokers, decks, or other game elements. They are an excellent way to test your adaptability and understanding of Balatro's mechanics.

Examples of challenge run types include:
- Specific Starting Jokers: Begin with a set of Jokers, forcing a particular build.
- Altered Shop Contents: Shops might only sell certain types of cards or Jokers.
- Unique Scoring Conditions: Score bonuses for specific actions or penalties for others.
- Limited Resources: Fewer discards, plays, or starting money.