The Inverted Castle features several new and challenging bosses, each guarding a Dracula Relic or a path forward. Here's a brief overview (detailed strategies are in the 'Boss Guides' section):

- Doppelganger40: Found in the Reverse Caverns. A more powerful version of the normal castle's Doppelganger.
- Death: Found in the Anti-Chapel. A classic Castlevania boss with scythe attacks and summoned skeletons.
- Medusa: Found in the Reverse Clock Tower. A giant Medusa head that petrifies and fires projectiles.
- Creature: Found in the Reverse Outer Wall. A massive, Frankenstein-like monster with powerful physical attacks.
- Galamoth: An optional, extremely powerful boss found in the Floating Catacombs. Defeating him grants a rare item.
Each boss requires careful strategy and often specific equipment. Ensure you are well-stocked with healing items and have your best gear equipped before engaging.