Mission 01: Assigning the First Mission in Metal Gear Solid: Peace Walker marks the official start of Militaires Sans Frontières' independent operations. After establishing their presence, Big Boss and Miller receive a request from a mysterious woman named Amanda, who claims her country is being threatened by an armed force. This mission serves as an introductory segment, guiding players through the basic controls and mechanics of the game, including movement, aiming, shooting, and the initial use of the Fulton Recovery System to capture enemy soldiers.

The objective of this mission is to infiltrate a small enemy outpost and gather intelligence on the encroaching armed force. utilize stealth to avoid detection, taking down guards silently and using cover effectively. The mission culminates in a brief encounter that highlights the dangers posed by the enemy. Successfully completing this mission not only progresses the story but also allows players to begin recruiting their first few soldiers for Mother Base, laying the groundwork for future expansions and upgrades.