These items protect you, boost your speed, or provide strategic advantages. Knowing when to deploy them can save your race.

- Mushroom: Provides a short burst of speed. Essential for taking shortcuts, recovering from spins, or gaining a quick lead. You start Time Trial with three Mushrooms, highlighting their importance for optimal racing lines.
- Star: Grants temporary invincibility, increased speed, and the ability to spin out any opponent you touch. Also makes you immune to environmental hazards like Thwomps, lava, and falling off rail-less tracks. A game-changer when obtained, especially on challenging tracks like Rainbow Road or Bowser Castle.
- Ghost: Steals an item from an opponent and makes you temporarily invisible and intangible. Useful for disarming a leading opponent or avoiding incoming attacks. The stolen item is immediately added to your inventory.
- Lightning Bolt: Shrinks all opponents, making them slower and more susceptible to being crushed. The smaller they are, the longer they stay shrunk. A powerful item for catching up from behind or solidifying a lead.
Defensive items are often best held until you need them, especially the Star or Ghost. A well-timed Lightning Bolt can completely change the dynamic of a race, especially in the final lap.